Enclosed is an example of a Biztalk Error that can be resolved by the application of an Microsoft .NET Framework 2.0 hotfix.

ENTSSO   

Error   

Enterprise Single Sign-On    

10514   

Server1   "An error occurred while attempting to access the SSO database.
Function: GetGlobalInfo
File: infocache.cpp:1349
System.Data : Timeout expired.  The timeout period elapsed prior to obtaining a connection from the pool.  This may have occurred because all pooled connections were in use and max pool size was reached..
SQL Error code: 0xE2E2E2E2
Error code: 0xC0002A21, An error occurred while attempting to access the SSO database.

http://support.microsoft.com/?id=948868